| 000 -LEADER |
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| fixed length control field |
03081nam a22001577a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
610 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Rehman ,Sannan ur |
| 245 ## - TITLE STATEMENT |
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| Title |
In vitro evaluation of antithrombotic and anticoagulant properties of Silymarin-Silver Nanoparticles using human blood samples / |
| Statement of responsibility, etc. |
Dr. Sannan ur Rehman |
| 264 ## - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Islamabad: |
| Name of producer, publisher, distributor, manufacturer |
SMME- NUST. |
| Date of production, publication, distribution, manufacture, or copyright notice |
2024. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
113p. ; |
| Other physical details |
Soft Copy, |
| Dimensions |
30cm. |
| 500 ## - GENERAL NOTE |
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| General note |
This study investigates the anticoagulant and antithrombotic properties of Silymarin Silver<br/>Nanoparticles (Sily-Ag NPs) using in vitro human blood samples. Silymarin, a natural flavonoid,<br/>was utilized for silver nanoparticles green synthesis, where it served as a reducing as well as a<br/>stabilizing agent. Comprehensive characterization of Sily-Ag NPs was done using UV-Vis<br/>spectroscopy, Fourier Transform Infrared spectroscopy (FTIR), X-ray Diffraction (XRD),<br/>Atomic Force Microscopy (AFM), Zeta potential analysis and Scanning Electron Microscopy<br/>(SEM). These analyses confirmed the nanoscale size, crystalline structure, stable surface charge,<br/>and effective capping by silymarin, suggesting the potential of these nanoparticles in biomedical<br/>applications.<br/>The study further evaluated the properties of antithrombosis and anticoagulation of Sily-Ag NPs<br/>using a series of coagulation assays, including Activated Partial Thromboplastin Time (aPTT),<br/>Fibrinogen levels, Prothrombin Time (PT), International Normalization Ratio (INR), and<br/>Thrombin time (TT) assessment. The results demonstrated that Sily-Ag NPs significantly<br/>prolonged PT, APTT, and INR, indicating a strong anticoagulant effect by interfering with both<br/>pathways of the coagulation cascade be it intrinsic or extrinsic. The findings also suggested that<br/>Sily-Ag NPs could effectively inhibit thrombin generation, a critical enzyme in the coagulation<br/>process. Interestingly, the Thrombin Time assay did not show any significant change, indicating<br/>that the nanoparticles do not directly interfere with concluding step of clot formation, the<br/>transformation of fibrinogen to fibrin. However, a modest reduction in fibrinogen levels was<br/>seen, which aligns with the overall anticoagulant and antithrombotic effects.<br/>The study concludes that Silymarin Silver Nanoparticles possess significant anticoagulant and<br/>antithrombotic properties, making them promising candidates for the development of new<br/>anticoagulant therapies. These nanoparticles demonstrated the ability to modulate multiple steps<br/>in the coagulation pathways without affecting the final fibrinogen-fibrin conversion, potentially<br/>offering a targeted therapeutic approach with fewer side effects compared to conventional<br/>anticoagulants. To determine Sily-Ag NPs' safety and effectiveness in clinical settings as well as<br/>to fully explore their therapeutic potential, more investigation is required, including in vivo<br/>experiments and clinical trials.<br/> |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MS Biomedical Sciences (BMS |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Supervisor: Dr. Nosheen Fatima Rana |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="http://10.250.8.41:8080/xmlui/handle/123456789/46685">http://10.250.8.41:8080/xmlui/handle/123456789/46685</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
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| Koha item type |
Thesis |
