Characterization of Blood-Derived Exosomes for Diagnostics Insights in Neurological Diseases / (Record no. 612948)

000 -LEADER
fixed length control field 02196nam a22001577a 4500
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 610
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Tahir, Javaria
245 ## - TITLE STATEMENT
Title Characterization of Blood-Derived Exosomes for Diagnostics Insights in Neurological Diseases /
Statement of responsibility, etc. Javaria Tahir
264 ## - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Islamabad:
Name of producer, publisher, distributor, manufacturer SMME- NUST. 2024;
Date of production, publication, distribution, manufacture, or copyright notice 2025.
300 ## - PHYSICAL DESCRIPTION
Extent 87p.
Other physical details Soft Copy,
Dimensions 30cm
500 ## - GENERAL NOTE
General note Exosomes, nanosized extracellular vesicles, are emerging as potential non-invasive<br/>biomarkers for diagnosing neurological disorders. In this study, we explored the isolation<br/>and characterization of blood-derived exosomes to assess their diagnostic relevance.<br/>Blood samples were collected from two groups: a control group of individuals without<br/>neurological disorders and a patient group diagnosed with various neurological<br/>conditions. Plasma was extracted using differential centrifugation to remove cellular<br/>debris, apoptotic bodies, and larger vesicles. Exosomes were isolated from the plasma<br/>using a polymer-based precipitation method optimized with PEG-6000 to achieve high<br/>yield and purity. The isolated exosomes were then characterized using Scanning Electron<br/>Microscopy (SEM) to verify their morphology and methylene blue staining to confirm<br/>their extracellular vesicular nature. After staining, RNA was extracted from the exosomes<br/>using TRIzol, followed by cDNA synthesis to analyze the molecular profile. Gel<br/>electrophoresis was performed to assess the quality of the cDNA, and RT-PCR was<br/>conducted to detect biomarkers associated with neurological disorders. These molecular<br/>analyses provided insights into the differences between the control and diseased groups,<br/>highlighting potential biomarkers for disease diagnosis. The results of this study promise<br/>valuable insights into the potential properties of blood-derived exosomes in advancing<br/>non-invasive diagnostics for neurological diseases by contributing to early detection<br/>strategies and paving the way for improved patient care through innovative diagnostic<br/>tools.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MS Biomedical Sciences (BMS)
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Supervisor : Dr. Saima Zafar
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://10.250.8.41:8080/xmlui/handle/123456789/50093">http://10.250.8.41:8080/xmlui/handle/123456789/50093</a>
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme
Koha item type Thesis
Holdings
Withdrawn status Permanent Location Current Location Shelving location Date acquired Full call number Barcode Koha item type
  School of Mechanical & Manufacturing Engineering (SMME) School of Mechanical & Manufacturing Engineering (SMME) E-Books 02/25/2025 610 SMME-TH-1116 Thesis
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