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In inertial Microfluidic field, movement of different particle causes different equilibrium
position in microchannel because of the inertial effects. For particle sorting this is not appreciable.
So with the help of secondary flow (which is at right angle to the primary flow) and drag of
particles, number of equilibrium positions can be reduced and this also help in focusing of the
particles. Amplitude and direction of secondary flow can be influenced by the curved configuration
of the microchannel. In this thesis work, three different size of blood cells (Erythrocyte 9µm,
Lymphocyte 15µm and Monocyte 20µm) were analyzed using passive techniques. We used two
different techniques for the manufacturing of microchannel. First microchannel was produced by
using SLA 3D printing and second channel was manufacture by using laser cutting technique. In
both techniques, there was only one inlet and three outlets. We use three different angles (30, 60
and 90) between outlets for each manufacturing technique. Both channels were studied under
microscope. Among these two channels laser channel gives better results. After taking images with
the help of microscope, image post processing was done by using MATLAB. We can use that
microfluidic device in medical labs as it is easy to use, portable and had low cost.