NUST Institutions Library Catalogue

One of the serious challenges in the treatment of infectious diseases is the presence of bacterial
infections in subcutaneous wound tissue. Staphylococcus epidermidis (S. epidermidis) and
Propionibacterium acne (P. acne) are resistant bacterial strains that cause severe disease in
humans when penetrating the deeper layer of skin. Antibacterial drugs with a nonspecific target
have more difficulty in penetrating the deeper layer of infected skin. Broad spectrum antibiotics
are best to treat the infection but are not commonly used because bacteria’s make resistance
against them. To overcome these issues, a combined strategy of broad-spectrum antibacterial
drug and nanoparticle was formulated for targeted delivery, enhanced penetration to the infection
site specifically. The ciprofloxacin was entrapped in transethosomes and formulation was
synthesized by using the cold method. Transethosomes are very small in size that can reach the
deeper layer of skin to give potential effects. In the layers of skin, ciprofloxacin works by
binding to the enzymes topoisomerase IV and DNA gyrase and inhibit the DNA replication in
bacteria. The antibacterial activity of ciprofloxacin loaded transethosomes against skin infections
was assessed using Staphylococcus epidermidis and Propionibacterium acne and the method
used was well diffusion method. The characterization of ciprofloxacin loaded transethosomes
was done through, zeta potential, particle size evaluation, and drug release efficiency. Loaded
transethosomes displayed substantially have more potential effect than ciprofloxacin alone. The
in-vitro studies show that ciprofloxacin loaded transethosomes boost the antibacterial activity of
ciprofloxacin against gram positive.