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Prevalence and Mechanism of Carbapenem-resistant Genes in Acinetobacter baumannii / Komal Khalid

By: Khalid, Komal Contributor(s): Supervisor : Dr. Amer Sohail KashifMaterial type: Text

TextIslamabad : SMME- NUST; 2022Description: 58p. Soft Copy 30cmSubject(s): MS Biomedical Sciences (BMS)DDC classification: 610 Online resources: Click here to access online

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Item type	Current location	Home library	Shelving location	Call number	Status	Date due	Barcode	Item holds
Thesis	School of Mechanical & Manufacturing Engineering (SMME)	School of Mechanical & Manufacturing Engineering (SMME)	E-Books	610 (Browse shelf)	Available		SMME-TH-727

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Coccobacilli with a gram-negative Acinetobacter baumannii is a member of the ESKAPE group
of free-living organisms, which are widely found in the environment in places like water, food,
soil, sewage, animals, and humans. It is an opportunistic pathogen that has been implicated in
numerous nosocomial infections across the globe. Carbapenem resistance has formed into a
potential threat to humanity because of its rising prevalence among healthcare facilities. The
present study was designed to characterize the presence of carbapenem-resistant genes including
multidrug-resistant Acinetobacter baumannii strains, isolated from the tertiary care hospital in
Pakistan. A biochemical test was performed for the initial identification of bacterial strains and
further confirmed by MALDI-TOF mass spectrometry. Antibiotic susceptibility testing was done
by using the standard Kirby-Bauer disk diffusion method, and carbapenem resistance determinants
were further screened Metallo-beta-lactamase manufacture through Imipenem/Imipenem-EDTACDT. All 48 A. baumannii strains were further amplified by PCR for detection of OXA-51-like,
OXA-23-like, and blaNDM genes. The OXA-51-like gene was detected in 46 strains (95.83%),
while OXA-23-like was detected in 44 (91.66%) strains of carbapenem-resistant Acinetobacter
baumannii. Moreover, New Dehli MBL, blaNDM gene was identified in four (8.33%) MBL
positive carbapenem-resistant Acinetobacter baumannii (CRAB) isolates. All 4 positive blaNDM
isolates were co-expressed by the OXA-23-like gene while OXA-51-like was absent in one
blaNDM positive strain of carbapenem-resistant Acinetobacter baumannii isolates. A high
prevalence of OXA-51-like, and OXA-23-like genes was seen among the carbapenem-resistant
Acinetobacter baumannii isolates, followed by blaNDM1. The results of the study revealed that
MDR Acinetobacter baumannii strains from two important cities in Pakistan (Rawalpindi and
Islamabad) had a significant presence of carbapenem-resistant genes. This finding may result in
clinical therapeutic failures.

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