Metal Nanoparticles Enhances Sorafenib Activity against Cancerous Cell Lines / Muhammad Ammad Jamil
Material type:
TextIslamabad : SMME- NUST; 2024Description: 108p. Soft Copy 30cmSubject(s): MS Biomedical Sciences (BMS)DDC classification: 610 Online resources: Click here to access online
| Item type | Current location | Home library | Shelving location | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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School of Mechanical & Manufacturing Engineering (SMME) | School of Mechanical & Manufacturing Engineering (SMME) | E-Books | 610 (Browse shelf) | Available | SMME-TH-1036 |
Globally, cancer prevalence is increasing rapidly, requiring urgent development of effective
therapeutics strategies. Cancer is characterized by uncontrolled cell growth and is categorized into
various stages and types, each with different epidemiology. Cancer cell lines are derived from
many tumor types, then cultured in vitro, and are essential for studying cancer genetic and drug
testing. Sorafenib is a multi-kinase inhibitor drug, widely used in treatment of hepatocellular
carcinoma and renal cell carcinoma, but has limitations such as low bioavailability and solubility.
This study deals with the use of bacopa monnieri for the synthesis of copper formulated and zinc
formulated nano-coated drugs; and investigates their effect of on cancer cell line (MCF-7). Both
nano-coated drugs were characterized by various techniques; UV-Vis spectrometry confirmed the
drug absorbance at 262nm and 263nm, Fourier transform infrared spectroscopy (FTIR) confirmed
the presence of interaction between the sorafenib drug and nanoparticles, X-ray diffraction (XRD)
confirmed their crystallinity nature, Scanning electron microscopy (SEM) revealed that copper
formulated nano-coated drug was stable with good coating and surface morphology and zinc
formulated nano-coated drug was less stable due to irregular shape morphology, Dynamic light
scattering (DSL) confirmed that copper formulated nano-coated drug size ranged from 12nm to
13nm and zinc formulated nano-coated drug size ranged from 9nm to 10nm, Zeta potential
confirmed that copper formulated nano-coated drug was stable (fit values ranged between (-21mV ̶
-18mV)) and zinc formulated nano-coated drug was slightly less stable (fit values ranges -7.12mV ̶
+12.35mV). MTT assay showed that copper formulated nano-coated drug has the cell viability of
64.8% with IC50 value of 4.599µM and zinc formulated nano-coated drug has the cell viability of
68.5% with IC50 value of 34.50µM. Both nano-coated drugs exhibited less cell viability than the
actual drug and are stable, thus, making them suitable for the cancer treatment.
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