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Diabetic wound (DW) healing remains a significant healthcare concern due to prolonged
healing times and high infection rates, leading to inadequate treatment outcomes for many
patients. Current therapies often fail to address the complex requirement of diabetic
wounds, necessitating novel approaches. This study explores the development of a thymolloaded hydrogel as an innovative therapeutic strategy to accelerate the wound healing and
prevent infections in patients with diabetes. Thymol, naturally occurring compound with
well-documented anti-inflammatory and antibacterial properties, was encapsulated in
liposomes to enhance its stability and ensure sustained release at the wound site. These
thymol-loaded liposomes were then incorporated into a hydrogel matrix, synthesized
through cross-linking polymerization optimized for stability, controlled release, and
maintaining a moist wound environment. The hydrogel’s antibacterial efficacy was
assessed using the well diffusion method against Staphylococcus aureus and Enterococcus
faecalis. Additionally, anti-biofilm assays demonstrated the hydrogel's ability to prevent
biofilm formation. In-vivo experiments conducted on a diabetic rat model revealed that
wounds treated with this hydrogel exhibited significantly faster healing and improved
tissue regeneration compared to untreated controls. These findings suggest that thymolloaded hydrogel represents a promising approach for managing diabetic wounds (DW),
offering both accelerated healing and effective infection control. This novel therapeutic
approach holds potential for clinical application, improving outcomes for diabetic patients suffering from chronic wounds.