Hameed, Farhan  <br/><br/>
Therapeutic Application of Crude Mitochondrial Transplantation for Diabetic Wound Healing / 
Farhan Hameed 
 - 77p.  Soft Copy  30cm 
<br/><br/> Chronic wounds in diabetic condition pose a persistent clinical challenge, primarily due<br/>to impaired cellular energy metabolism and delayed tissue regeneration. Mitochondrial<br/>dysfunction plays pivotal role in this impaired healing process, making mitochondrial<br/>transplantation (MT) a promising therapeutic strategy. This study investigates the<br/>efficacy of MT in accelerating wound repair using a diabetic mice model. Full-thickness<br/>excisional wounds (6mm) were created on the dorsal surface of diabetic mice, followed<br/>by subcutaneous administration of isolated crude mitochondria at low, moderate and<br/>high dose (5ug, 10ug, and 20ug) mitochondria per wound. A vehicle-treated group<br/>receiving phosphate-buffered saline (PBS) served as the control.<br/>Mitochondrial viability and quantification were assessed using MTT assay and Bradford<br/>in wound contraction. Wound closure analysis revealed a dose-dependent acceleration of<br/>healing, with high-dose group exhibiting the most significant improvement in wound<br/>contraction and re-epithelialization. Histological analysis confirmed enhanced tissue<br/>regeneration in the treatment groups. Furthermore real-time PCR analysis demonstrated a<br/>significant upregulation of RAC1, CDC42, and VEGF in the moderate and high treatment<br/>groups, indicating enhanced cytoskeletal remodeling, cellular migration and<br/>angiogenesis.<br/>These findings highlight the therapeutic potential of crude mitochondrial transplantation<br/>in diabetic wound healing by restoring cellular bioenergetics and promoting tissue repair.<br/>This approach offers a promising regenerative strategy for managing chronic wound and<br/>improving diabetic wound outcomes. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>MS Biomedical Sciences (BMS)
<br/><br/><label>Dewey Class. No.: </label>610