000 02221nam a22001577a 4500
082 _a610
100 _aHameed, Farhan
_9130434
245 _aTherapeutic Application of Crude Mitochondrial Transplantation for Diabetic Wound Healing /
_cFarhan Hameed
264 _aIslamabad :
_bSMME- NUST;
_c2025.
300 _a77p.
_bSoft Copy
_c30cm
500 _aChronic wounds in diabetic condition pose a persistent clinical challenge, primarily due to impaired cellular energy metabolism and delayed tissue regeneration. Mitochondrial dysfunction plays pivotal role in this impaired healing process, making mitochondrial transplantation (MT) a promising therapeutic strategy. This study investigates the efficacy of MT in accelerating wound repair using a diabetic mice model. Full-thickness excisional wounds (6mm) were created on the dorsal surface of diabetic mice, followed by subcutaneous administration of isolated crude mitochondria at low, moderate and high dose (5ug, 10ug, and 20ug) mitochondria per wound. A vehicle-treated group receiving phosphate-buffered saline (PBS) served as the control. Mitochondrial viability and quantification were assessed using MTT assay and Bradford in wound contraction. Wound closure analysis revealed a dose-dependent acceleration of healing, with high-dose group exhibiting the most significant improvement in wound contraction and re-epithelialization. Histological analysis confirmed enhanced tissue regeneration in the treatment groups. Furthermore real-time PCR analysis demonstrated a significant upregulation of RAC1, CDC42, and VEGF in the moderate and high treatment groups, indicating enhanced cytoskeletal remodeling, cellular migration and angiogenesis. These findings highlight the therapeutic potential of crude mitochondrial transplantation in diabetic wound healing by restoring cellular bioenergetics and promoting tissue repair. This approach offers a promising regenerative strategy for managing chronic wound and improving diabetic wound outcomes.
650 _aMS Biomedical Sciences (BMS)
700 _aSupervisor : Dr. Hussain Mustatab Wahedi
_9130435
856 _uhttp://10.250.8.41:8080/xmlui/handle/123456789/54551
942 _2ddc
_cTHE
999 _c614596
_d614596